Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007-2008 influenza season in Toronto, Canada.
Identifieur interne : 000663 ( Main/Exploration ); précédent : 000662; suivant : 000664Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007-2008 influenza season in Toronto, Canada.
Auteurs : Shelly Bolotin [Canada] ; Ernesto Lombos ; Rani Yeung ; Alireza Eshaghi ; Joanne Blair ; Steven J. DrewsSource :
- Virology journal [ 1743-422X ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A.
- virologie : Grippe humaine.
- Humains, Ontario, Saisons, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- geographic : Ontario.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype.
- virology : Influenza, Human.
- Humans, Seasons, Sensitivity and Specificity.
Abstract
The increase in adamantine resistance in influenza A (H3N2) and the emergence of oseltamivir resistance in influenza A (H1N1) has necessitated the use of rapid methodologies to detect influenza subtype. The purpose of this study was to evaluate the CombiMatrix influenza detection system compared to the FDA approved Luminex Respiratory virus panel (RVP) assay for influenza A subtyping. Verification of the CombiMatrix influenza detection system was carried out using the Luminex RVP assay as a reference method. A limit of detection (LOD) series was performed using the Luminex and CombiMatrix systems with both influenza A H3N2 and H1N1 viruses. Seventy-five clinical specimens were used in the study. Of these, 16 were influenza A (H3N2) positive and five were influenza A (H1N1) positive. Fifty-four specimens were influenza A negative or "no call" (inconclusive) or could not be subtyped. The LOD of the Luminex RVP assay was found to be 0.3 TCID50s/mL for influenza A (H3N2) and 16 TCID50s/mL for influenza A (H1N1). The LOD of the CombiMatrix influenza detection system was 200 TCID50s/mL for influenza A (H3N2) and 16 000 TCID50s/mL for influenza A (H1N1). The sensitivity of the CombiMatrix influenza detection system was 95.2% and the specificity was 100%. The CombiMatrix influenza detection system is an effective methodology for influenza A subtype analysis, specifically in laboratories with a constrained budget or limited molecular capabilities.
DOI: 10.1186/1743-422X-6-37
PubMed: 19321005
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Shelly.bolotin@oahpp.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ontario Agency for Health Protection and Promotion, 81 Resources Road, Toronto, Ontario, M9P 3T1</wicri:regionArea><wicri:noRegion>M9P 3T1</wicri:noRegion></affiliation></author><author><name sortKey="Lombos, Ernesto" sort="Lombos, Ernesto" uniqKey="Lombos E" first="Ernesto" last="Lombos">Ernesto Lombos</name></author><author><name sortKey="Yeung, Rani" sort="Yeung, Rani" uniqKey="Yeung R" first="Rani" last="Yeung">Rani Yeung</name></author><author><name sortKey="Eshaghi, Alireza" sort="Eshaghi, Alireza" uniqKey="Eshaghi A" first="Alireza" last="Eshaghi">Alireza Eshaghi</name></author><author><name sortKey="Blair, Joanne" sort="Blair, Joanne" uniqKey="Blair J" first="Joanne" last="Blair">Joanne Blair</name></author><author><name sortKey="Drews, Steven J" sort="Drews, Steven J" uniqKey="Drews S" first="Steven J" last="Drews">Steven J. 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Drews</name></author></analytic><series><title level="j">Virology journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza A Virus, H3N2 Subtype (isolation & purification)</term><term>Influenza, Human (virology)</term><term>Ontario</term><term>Seasons</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Grippe humaine (virologie)</term><term>Humains</term><term>Ontario</term><term>Saisons</term><term>Sensibilité et spécificité</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Sous-type H3N2 du virus de la grippe A (isolement et purification)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Ontario</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Seasons</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Ontario</term><term>Saisons</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The increase in adamantine resistance in influenza A (H3N2) and the emergence of oseltamivir resistance in influenza A (H1N1) has necessitated the use of rapid methodologies to detect influenza subtype. The purpose of this study was to evaluate the CombiMatrix influenza detection system compared to the FDA approved Luminex Respiratory virus panel (RVP) assay for influenza A subtyping. Verification of the CombiMatrix influenza detection system was carried out using the Luminex RVP assay as a reference method. A limit of detection (LOD) series was performed using the Luminex and CombiMatrix systems with both influenza A H3N2 and H1N1 viruses. Seventy-five clinical specimens were used in the study. Of these, 16 were influenza A (H3N2) positive and five were influenza A (H1N1) positive. Fifty-four specimens were influenza A negative or "no call" (inconclusive) or could not be subtyped. The LOD of the Luminex RVP assay was found to be 0.3 TCID50s/mL for influenza A (H3N2) and 16 TCID50s/mL for influenza A (H1N1). The LOD of the CombiMatrix influenza detection system was 200 TCID50s/mL for influenza A (H3N2) and 16 000 TCID50s/mL for influenza A (H1N1). The sensitivity of the CombiMatrix influenza detection system was 95.2% and the specificity was 100%. The CombiMatrix influenza detection system is an effective methodology for influenza A subtype analysis, specifically in laboratories with a constrained budget or limited molecular capabilities.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19321005</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1743-422X</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2009</Year><Month>Mar</Month><Day>25</Day></PubDate></JournalIssue><Title>Virology journal</Title><ISOAbbreviation>Virol. J.</ISOAbbreviation></Journal><ArticleTitle>Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007-2008 influenza season in Toronto, Canada.</ArticleTitle><Pagination><MedlinePgn>37</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1743-422X-6-37</ELocationID><Abstract><AbstractText>The increase in adamantine resistance in influenza A (H3N2) and the emergence of oseltamivir resistance in influenza A (H1N1) has necessitated the use of rapid methodologies to detect influenza subtype. The purpose of this study was to evaluate the CombiMatrix influenza detection system compared to the FDA approved Luminex Respiratory virus panel (RVP) assay for influenza A subtyping. Verification of the CombiMatrix influenza detection system was carried out using the Luminex RVP assay as a reference method. A limit of detection (LOD) series was performed using the Luminex and CombiMatrix systems with both influenza A H3N2 and H1N1 viruses. Seventy-five clinical specimens were used in the study. Of these, 16 were influenza A (H3N2) positive and five were influenza A (H1N1) positive. Fifty-four specimens were influenza A negative or "no call" (inconclusive) or could not be subtyped. The LOD of the Luminex RVP assay was found to be 0.3 TCID50s/mL for influenza A (H3N2) and 16 TCID50s/mL for influenza A (H1N1). The LOD of the CombiMatrix influenza detection system was 200 TCID50s/mL for influenza A (H3N2) and 16 000 TCID50s/mL for influenza A (H1N1). The sensitivity of the CombiMatrix influenza detection system was 95.2% and the specificity was 100%. The CombiMatrix influenza detection system is an effective methodology for influenza A subtype analysis, specifically in laboratories with a constrained budget or limited molecular capabilities.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bolotin</LastName><ForeName>Shelly</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Ontario Agency for Health Protection and Promotion, 81 Resources Road, Toronto, Ontario, M9P 3T1, Canada. 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DateType="Electronic"><Year>2009</Year><Month>03</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Virol J</MedlineTA><NlmUniqueID>101231645</NlmUniqueID><ISSNLinking>1743-422X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000821" 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Drews</name><name sortKey="Eshaghi, Alireza" sort="Eshaghi, Alireza" uniqKey="Eshaghi A" first="Alireza" last="Eshaghi">Alireza Eshaghi</name><name sortKey="Lombos, Ernesto" sort="Lombos, Ernesto" uniqKey="Lombos E" first="Ernesto" last="Lombos">Ernesto Lombos</name><name sortKey="Yeung, Rani" sort="Yeung, Rani" uniqKey="Yeung R" first="Rani" last="Yeung">Rani Yeung</name></noCountry><country name="Canada"><noRegion><name sortKey="Bolotin, Shelly" sort="Bolotin, Shelly" uniqKey="Bolotin S" first="Shelly" last="Bolotin">Shelly Bolotin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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